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. 2019 May 28;294(28):10886–10899. doi: 10.1074/jbc.RA118.005385

Figure 3.

Figure 3.

Overexpression of CD2AP reduces the localization of APP in early endosomes and increases localization in late endosomes. a, effect of CD2AP on the colocalization of APP with EEA1 or Rab7, markers for early endosomes or late endosomes. Myc-APP was transfected into HEK293 cells in the absence (−) or presence (+) of CD2AP. APP, EEA1, and Rab7 were immunostained with anti-Myc, anti-EEA1, and anti-Rab7 antibodies, respectively. APP is shown in the left, EEA1 or Rab7 in the 2nd, and the merge in the 3rd panels. The right panels are a higher magnification view of the white box in the merges. The X-Z and Y-Z reconstruction along the white lines of the X-Y image is shown at the upper and right side of each panel, respectively. Bars for the left three panels, 20 μm; right panels, 5 μm. b, percentage of APP colocalization with EEA1 or Rab7. Data are expressed as the mean ± S.E. (n = 20 for EEA1 and Rab7). c, relative level of APP colocalized with EEA1 or Rab7 in the presence or absence of CD2AP (mean ± S.E. n = 20 for EEA1 and Rab7. *, p < 0.05, **, p < 0.01, Student's t test).