Skip to main content
. 2019 May 28;294(28):10833–10845. doi: 10.1074/jbc.RA118.007083

Figure 1.

Figure 1.

Differential binding of Siglec-7–Fc to GD3. A, flow cytometry analysis. Green lines indicate expression levels of GD3 on parent DLD-1 and GD3 synthase cDNA-transfected DLD-1 GD3S as analyzed by anti-GD3 mAb (R24). Purple lines indicate Siglec-7–Fc-binding levels. B, acidic glycolipids were isolated from DLD-1 GD3S using DEAE-Sephadex A-50TM. Resorcinol spray was performed to detect sialic acids on gangliosides. BBG, bovine brain gangliosides as a control. C, bovine milk-derived GD3 was added to DLD-1 culture medium at 12.5 mm. After 5 h, cells were analyzed with FCM for ganglioside expression (anti-GD3 and anti-GD2 mAbs) and Siglec-7–Fc binding. D, TLC-immunostaining was performed with mAb R24 or Siglec-7–Fc.