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. 2019 May 28;294(28):10833–10845. doi: 10.1074/jbc.RA118.007083

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© 2019 Hashimoto et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 4. — Analysis of bovine milk- and colon cancer cell–derived ganglioside GM3. A, as shown in the case of GD3, monosialyl ganglioside GM3 was analyzed. Bovine milk GM3 had saturated ceramide moiety and major peaks of DLD-1–derived GM3 were hydroxylated as GD3. B and C, ganglioside profiles of colon cancer cell lines. Gangliosides were isolated from colon cancer cell lines WiDr and Caco-2 and analyzed by MS (B), and also by TLC-resorcinol with those of DLD-1 and DLD-1 GD3 S (C). M, bovine brain ganglioside mixture.