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. 2019 Jul 16;9:10304. doi: 10.1038/s41598-019-46623-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Confirmation of H1 in Human Tears using Multiple reaction monitoring (MRM). The MRM was operated in the positive ion mode monitoring the 704.9–931.0 m/z transitions for H1 to identify the presence of H1 in saliva, tears and a positive control solution containing synthetic H1. Shown are representative results of MRM testing of tears of two normal (non DED) patients. Similar results were found with MRM testing in three separate experiments.