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. 2019 Jul 16;9:10318. doi: 10.1038/s41598-019-46560-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Kinetics of simultaneous metal ion removal. (A) Intracellular mercury accumulation (arrows) and deposition of metallic mercury nanospheres (M) by reduction of ionic mercury. (B) Similar kinetics of residual Hg after treatment of EH8’s activated sporangiospores with low (1 mg.L⁻¹) and high (50 mg.L⁻¹) concentrations⁵. (C) Similar kinetics of intracellular-fixed Hg at low (1 mg.L⁻¹) and high (50 mg.L⁻¹) concentrations. (D-F) Simultaneous removal of a metal mixture (>81–99% of Al, Cd, Co, Cr, Cu, Hg, Ni, Pb, U and Zn) by activated cell walls (CW, D), by activated spore mix (Sp, E) and by mixed microbiome (biofilm, Bf) grown on expanded clay spheres (F) of EH8, EH10 and EH11 (Fig. C.2) as well as enrichment of Au, Ag and Ti (Table 3). The fitted curve parameters with statistical significance are given in Table 2. Standard deviations of measurements (n = 3) at each data point were less than 5%.