Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 16;9:10276. doi: 10.1038/s41598-019-46659-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effects of transient overexpression of hnRNPA1 or hnRNPU on TRA2B expression. (a) Full-length hnRNPA1 mRNA was prepared and cloned into a pEBMulti vector. HCT116 cells were transfected with this vector for 48 h. The amounts of hnRNPA1 mRNA were measured by RT-qPCR. Fold-changes were calculated by comparing with the values of the empty vector-transfected cells (means ± SD, n = 4). (b,c) Levels of TRA2β1 and TRA2β4 were measured by RT-qPCR in HCT116 overexpressing hnRNPA1 or transfected with an empty vector. GAPDH mRNA was used as an internal control. Values are expressed as fold-changes compared with those in the empty vector-transfected cells (means ± SD, n = 4). *Significantly different by two-tailed Student’s t-test (p < 0.05). (d) After transfection with the pEBMulti vector carrying full-length hnRNPU mRNA or an empty vector, HnRNPU mRNA levels in the cells were measured by RT-qPCR. Values are means ± SD, n = 4. (e,f) TRA2β1 and TRA2β4 levels in the hnRNPU-overexpressing cells were measured by RT-qPCR and compared with those in the empty vector-transfected, control cells. Values are means ± SD, n = 4. *Significantly different by two-tailed Student’s t-test (p < 0.05). (g,h) Tra2β, hnRNPA1, and hnRNPU in hnRNPA1- or hnRNPU-overexpressing cells and control cells were measured by Western blot analysis.