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. 2019 Jul 16;10(4):e00808-19. doi: 10.1128/mBio.00808-19

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Copyright © 2019 Sangaré et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — Immunoprecipitation of TREX-293 GRA15_II and RH-GRA15_II. TREX-293 GRA15_II and ROP38_I were induced with tetracycline (1 μg/ml), harvested 16 h postinduction (A, B), or infected for 8 h with RH parasites expressing HA-tagged GRA15 (C). The cells were lysed for HA immunoprecipitation with magnetic beads. Immunoblotting was performed on 10% input and 30 μl of the HA magnetic beads with (A) HA antibody, (B) TRAF2, TRAF3, and TRAF6 antibodies, or (C) HA, TRAF2, and TRAF6 antibodies and lamin A as a loading control of the 10% input.