Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 16;10(4):e01083-19. doi: 10.1128/mBio.01083-19

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Trebosc et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

PMC Copyright notice

FIG 2 — Discrimination and quantification of pmrC and eptA. (A) Schematic representation of differences in the pmrC, eptA-1, and eptA-2 coding sequence. Primers marked by black (oVT162/oVT163) and red (oVT164/oVT165) arrows were used to detect pmrC and eptA in qRT-PCR experiments, respectively. The primers marked by green arrows (oVT152/oVT153) were used to genotype the eptA isoforms. Primers marked by blue (oVT198/oVT199) and orange (oVT201/oVT202) arrows were used to discriminate eptA-1 and eptA-2, respectively. (B) Expression levels of eptA were quantified by qRT-PCR in colistin-resistant A. baumannii isolates BV94 and BV189 (white bars) and their ΔpmrA mutants (black bars). The expression levels were normalized to the pmrC expression in the ATCC 17978 reference strain.