TABLE 1.
UPLC-QTOF-MS analysis of muropeptides from W. chondrophila PG (Fig. 1)
| Peak no. |
Retention time (min) |
% content |
[M + H]+
(calculated) |
[M + H]+
(observed) |
Mass difference (cal/obs)a |
Muropeptide |
|---|---|---|---|---|---|---|
| 1 | 4.94 | 23.31 | 942.4147 | M4b | ||
| 2 | 6.24 | 5.82 | 1,780.7591 | 1,780.7558 | 0.0033 | D33G |
| 3 | 6.37 | 4.88 | 1,723.7376 | 1,723.7349 | 0.0027 | D33 |
| 4 | 6.53 | 3.07 | 1,851.7962 | 1,851.8025 | 0.0063 | D43G/D34G |
| 5 | 6.65 | 3.6 | 1,794.7747 | 1,794.7693 | 0.0054 | D43 |
| 6 | 6.81 | 13.36 | 1,794.7747 | 1,794.7741 | 0.0006 | D34 |
| 7 | 7.11 | 25.79 | 1,865.8118 | 1,865.7996 | 0.0122 | D44 |
a
cal/obs, calculated/observed.
b
The identity of M4 was established by retention time matching with an E. coli PG reference (MS previously characterized) under the same separation conditions.