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. 2019 Jul 16;10(4):e01464-19. doi: 10.1128/mBio.01464-19

FIG 5.

FIG 5

TrpA amino acid alignment and phylogeny of C. trachomatis clinical strains F I-IV, A/HAR13, F/IC-Cal3, and Protein Data Bank (PDB) templates. (A) For 3D model reconstructions, amino acid alignments of Ct clinical strains F/I-IV, F/IC-Cal3, and A/HAR13 with the PDB templates of LBCA 5ey5, M. tuberculosis 5tch, and S. Typhimirium 1qopA was performed using Clustal Omega (see Materials and Methods). Residues involved in both catalytic and subunit interaction (magenta), α-L6 residues α176-196 according to the 5tch sequence (cyan), clinical strain F mutation G253D and .254N and .255L aa elongation (yellow), gray, basic amino acids arginine and lysine in template sequences (gray), and acidic amino acid aspartic acid in clinical strains (green) are indicated. (B) Phylogenetic tree of Ct strains and PDB aa templates using neighbor joining with Jones-Taylor-Thornton (JTT) substitution method with 10,000 bootstrap replications using MEGA 7 (see Materials and Methods).