TABLE 2.
Primers used in this study
| Primer name | Sequence 5′–3′a |
|---|---|
| pDLrocAFLAG_F | GGCCCTAGGTGATGTTAAAGGTATGAA |
| pDLrocAFLAG_R | GGCCCTAGGCTTGTCGTCATCGTCTTTGTAGTCGTCAGGCTTAGCTATTTC |
| pDL_rocAM3_F | CGGGATCCTTGCAAAAACTGTAGGCTGTG |
| pDL_rocAM3_R | CGGGATCCTTACTTGTCGTCATCGTCTTTGTAGTCAGTCAAATTGCCAGTC |
| pDL_rocATMD_FLAG_F | GGCCCTAGGTGAAACTCTATTGAGGCAATTG |
| pDL_rocATMD_FLAG_R | GGCCCTAGGCTTGTCGTCATCGTCTTTGTAGTCTTGTTTTACATAACGCTC |
| pDL_rocACYT_FLAG_F | GGAATTCCATATGAACTCTATTGAGGCAATTGTGC |
| pDL_rocACYT_FLAG_R | GGAATTCCATATGTTATCCTTCTCCTTCTCGTTC |
| chFLAG_1F | CGGGATCCGAATGCTGAAAAGAATAATGC |
| chFLAG_1R | TCACCTAGGCTTGTCGTCATCG |
| chFLAG_2F | GATGACGACAAGCCTAGGTGATGTTAAAGGTATGAA |
| chFLAG_2R | CGCCGTCGACCCCAACACCTGAAGCCCAGTG |
| L_csrS_F | CGGGATCCGAAAATCGAGATGTGATAA |
| L_csrS_R | CTCACGAATAACGTATCACCTAGGCTTGTCGTCATC |
| L_rocA_F | GACAAGCCTAGGTGATACGTTATTCGTGAGAAATA |
| L_rocA_R | CTAATGATGATGATGATGATGCTCTCTTTAGACTGGGCC |
| L_rocA_R_PstI | CGCCCTGCAGCTAATGATGATGATGATG |
| pET_ATPase_NcoI_F | CATGCCATGGAAAAGAATAATGCTAAAGATG |
| M1_pET_ATPase_HIS_R | CGGGATCCTCAATGATGATGATGATGATGGTCAGGCTTAGCTATTTC |
| M3_pET_ATPase_HIS_R | CGGGATCCTCAATGATGATGATGATGATGAGTCAAATTGCCAGTCATC |
| RocA2H_F | AAGGAAAAAAGCGGCCGCAAACTCTATTGAGGCAATTGTGC |
| RocA2H_R | CGGGATCCTCAGTCAGGCTTAGCTATTTC |
| M3RocA2H_R | CGGGATCCTTAAGTCAAATTGCCAGTCAT |
| CsrS T284A F | CATGAATTACGAGCGCCGGTTGCG |
| CsrS T284A R | CGCAACCGGCGCTCGTAATTCATG |
| BamHI-csrS-5' | CCCCGGATCCATGGAAAATCAGAAACAAAAACAG |
| csrS-ID-3'-SalI | CCCCGTCGACTTACTAACTCTCTTTAGACTGGGC |
a
Bold with underlining indicates restriction endonuclease sites.