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. 2019 Jul 10;10:1521. doi: 10.3389/fmicb.2019.01521

Figure 3.

Figure 3

The abiotic and biotic factors related to the relative abundance of bacterial phyla. These factors were identified with random forest (A) and redundancy analysis (RDA, B). For abiotic factors, we considered water depth (depth), total phosphorus (TP.water), total nitrogen (TN.water), conductivity (Con.sur) and concentrations of HCO3 (HCO3·water) and SiO2 (SiO2·water), pH (pH.bot and pH.sur), dissolved oxygen (DO.bot), sediment porosity, loss-on-ignition (LOI), the first two axes of metal ion principal component analysis (Comp.1 and Comp.2) and grain size. Grain size: <4 μm (GSL4), 4–16 μm (GS4_16), 32–64 μm (GS32_64), and > 64 μm (GSL64). Biotic factors included the biomass (Dia.bio and Chi.bio) and richness (Dia.rich and Chi,rich) of diatoms and chironomids, and chlorophyll a (Chla.bot and Chla.sur). For RDA, the abiotic variables were automatically selected based on Monte Carlo permutation tests (999 permutations).