Figure 4.

IFN-γ and TNF-α expression is reduced when healthy MAIT cells are activated in the presence of OAC tumor conditioned media. PBMC from n = 4 healthy donors received either medium only or TCR and cytokine stimulation in the absence and presence of tumor conditioned medium (TCM) from n = 6 stage III OAC patients, and intracellular cytokine production was measured by flow cytometry, as shown for a single representative donor (A). Percentage IFN-γ and TNF-α expression is shown within the MAIT (B,C), T cell (D,E), and CD8⁺ T cell (F,G) compartments, respectively. Datasets were analyzed by ANOVA (Kruskal-Wallis test followed by Dunn's Multiple Comparison Test). Horizontal bars indicate median values. *p < 0.05.