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. 2019 Jul 10;9:222. doi: 10.3389/fcimb.2019.00222

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Copyright © 2019 Shi, Wang, Quan, Yang, Shang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hypovirus infection and rapamycin treatment resulted in the accumulation of autophagosome-like vesicle. (A) Transmission electron micrographs of the hyphae. The hypha morphology of EP155 and EP155/CHV1-EP713 did not show apparent distinction. However, the intracellular structure of EP155/CHV1-EP713 showed much more membrane structures. Arrow indicates autophagosome-like vesicle. (B) The quantification of the average number of autophagosome-like vesicles per cell and a minimum of 20 cells were counted. Rapamycin-treated EM graphs (14 days on rapamycin-supplemented PDA plate). ^** indicates P < 0.001, determined by Student's t-test.