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. 2019 Jul 10;10:762. doi: 10.3389/fphar.2019.00762

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Copyright © 2019 Liu, Shao, Zhang, Yang, Li, Cao and Huang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reactive oxygen species (ROS) generation in HepG2 cells incubated with C3. (A, B) After incubated with C3 (0, 20, and 40 µg/ml) for 24 h, the levels of intracellular ROS were detected by DCFH-DA (2′,7′-Dichlorodihydrofluorescein diacetate) assay. (C) The levels of ROS were presented as fold change compared to the levels in the control group. The data were expressed as mean ± S.D. (n = 3). **P < 0.01, ***P < 0.001 compared to the control group.