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. 2019 Jul 9;10:690. doi: 10.3389/fphar.2019.00690

Figure 4.

Figure 4

Nobiletin inhibits the SRC/AKT pathway, STAT3, and YY1AP1 activation, and induces apoptosis-related protein expression. ACHN and Caki-2 cells were treated with different concentrations of nobiletin (0, 80, or 120 µM for ACHN; 0, 40, and 80 µM for Caki-2) for 24 h (A–C) or 48 h (D). The levels of phosphorylated AKT, phosphorylated STAT3, phosphorylated SRC, and the YY1AP1 protein were decreased, whereas the level of phosphorylated YY1AP1 was increased (A–C). The levels of BAX, cleaved caspase 3, and cleaved caspase 9 were increased, whereas that of BCL2 was decreased (D). Protein levels were examined by Western blot. Beta-actin was used as a control. Data are presented as means ± SD. *P < 0.05, **P < 0.01.