Figure 1.

Effects of lenalidomide on plasmacytoid dendritic cell survival. Human plasmacytoid dendritic cells (pDCs) were incubated for 24 h with the indicated concentrations of lenalidomide (LEN), bortezomib, or vehicle in the presence of 3 μM CpG-ODN 2216. After 24 h, viable cells were measured by annexin V staining and flow cytometry. Percentages of annexin V-positive cells are indicated. Data are shown as means ± SEMs of six independent donors. Statistical significance was determined using paired Student’s t-tests (*=p<0.05, **=p<0.01; p-values were calculated for each concentration of LEN or bortezomib versus vehicle control).