Figure 3.

Action of MIM on neurons survival. Cultures of primary neurons treated for 1 hr with MIM and its placebo at (A) 10 μM and (B) 10 mM or BDNF (3.3 μM) and then injured by 6-OHDA (20 μM) for 48 hrs (results expressed as percentage of control). 6-OHDA induced a decrease of neuronal survival ((A) 65.32% and (B) 64.55% of the control). BDNF (3.3 μM; positive control) reduced 6-OHDA-induced cell-death ((A) 93.16% and (B) 91.26% of the control). MIM prevented 6-OHDA injury ((A) 89.84% and (B) 97.61% of the control). Quantification represents mean±S.D. of 6 wells per condition ((A) n=3 and (B) n=2); *p<0.05, **p<0.01, ***p<0.005, ^#p<0.0001; control, placebo (10 μM and 10 mM), MIM (10 μM and 10 mM) and BDNF (3.3 μM) vs 6-OHDA (20 μM); one-way ANOVA followed by Dunnett’s test; placebo (10 μM and 10 mM) vs MIM (10 μM and 10 mM); MIM (10 μM and 10 mM) vs BDNF (3.3 μM); Bonferroni’s multiple comparison test.

Abbreviations: 6-OHDA, 6-hydroxydopamine; MIM, micro-immunotherapy medicine; BDNF, brain-derived neutrophic factor.