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MRM transitions used1
| Vitamin D | MW (mol) | MRM transition (m/z) |
|---|---|---|
| Cholecalciferol | ||
| VD3 | 384.64 | 591.2/298.1 |
| 13C-VD3 (IS) | 389.60 | 596.2/298.1 |
| Calcifediol | ||
| 25(OH)D3 | 400.65 | 607.2/298.1 |
| 13C-25(OH)2D3 (IS) | 405.59 | 612.2/298.1 |
| Calcitriol | ||
| 1,25(OH)2D3 | 416.64 | 623.4/314.1 |
| d6–1,25(OH)2D3 (IS) | 422.67 | 629.4/314.1 |
IS, internal standard; MRM, multiple reaction monitoring; MW, molecular weight; VD3, vitamin D3; 25(OH)D3, 25-hydroxyvitamin D3; 1,25(OH)2D3, 1,25-dihydroxyvitamin D3.
