PcG Activity Is Required for the Stable Silencing of WUS.
(A) The wild-type flower.
(B) and (C) Indeterminate phenotypes of flowers of the Pro35S:GFP-FIE cosuppression line showing multiple carpels (B) and an internal ectopic carpelloid organ (red arrowhead) within the primary carpels (C).
(D) to (F) Through in situ hybridization, expression of WUS was not detected in stages 6 (D), 7 (E), and 10 (F) wild-type flower buds. WT, wild type.
(G) to (I) Prolonged WUS expression in FIE-silenced line Pro35S:GFP-FIE flower buds at stages 6 (G), 7 (H), and 10 (I). Black arrowheads show ectopic WUS expression in meristematic cells. Bars = 1 mm in (A) to (C); 50 µm in (D) to (I).