Spatial Localization and Diffusion Dynamics of Carboxysomes in Syn7942 Cells Are Dependent on Light Intensity.
(A) Tracking of carboxysome diffusion in cells grown under HL, ML, and LL. Colored lines indicate the diffusion trajectories of each carboxysomes, and circles represent the diffusion areas of each carboxysomes over 60 min. Scale bar = 1 μm.
(B) Nonlinear MSD vs. the time interval (𝛕) profiles suggest the mobility of carboxysomes in Syn7942 cells grown under HL, ML, and LL. Inset, zoom-in view of the MSD profile under LL.
(C) Diffusion coefficient of carboxysomes in vivo decreases significantly when the light intensity reduces: 2.76 ± 2.83 × 10−5 µm2·s−1 for HL (mean ±sd, n = 105), 1.48 ± 1.03 × 10−5 µm2·s−1 for ML (n = 84), and 0.28 ± 0.19 × 10−5 µm2·s−1 for LL (n = 336). P = 3.05 × 10−5 between HL and ML; P = 2.77 × 10−5 between ML and LL, two-tailed Student’s t test, *** P < 0.005.