Table 1.

Host species, dilution, and supplier information for primary antibodies used in IHC experiments

1° Antibody	Host	Supplier	Dilution	Specificity/control
ADIPOR1	Rabbit	Dr. Xiao-Rong Peng	1:200	Staining absent in western blot of Adipor1 KO mouse brain tissue (Bjursell et al. 2007)
ADIPOR2	Rabbit	Dr. Xiao-Rong Peng	1:200	Staining absent western blot of Adipor2 KO mouse brain tissue (Bjursell et al. 2007)
T-cadherin	Goat	R&D systems (Minneapolis, MA, U.S.A. AF3264) RRID: AB_2077121	1:500	Signal absent in IHC staining of T-Cad KO mouse cardiac tissue (Denzel et al. 2010)
NTPDase2	Rabbit	J. Sévigny, (Université Laval, Quebec, Canada. #mN2-36I6) RRID: AB_2314986	1:500	Specificity demonstrated in COS-7 cells and staining patterns coincide with RNA patterns in taste tissue (Bartel et al. 2006)
PLCβ2	Rabbit	Santa Cruz Biotechnologies (Dallas, TX, U.S.A. cat No. sc-206) RRID: AB_632197	1:500	Staining absent when primary or secondary omitted, validated in taste tissue (Yang et al. 2007)
Gα gustducin	Rabbit	Santa Cruz Biotechnologies (Dallas, TX, U.S.A. cat No. sc-395) RRID: AB_673678	1:500	Staining absent when primary or secondary omitted, validated in taste tissue (Yang et al. 2007)
NCAM	Rabbit	Millipore (Temecula, CA, USA; cat. No. AB5032) RRID: AB_2291692	1:500	Specificity confirmed by western blot of mouse brain homogenates and validated in taste tissue (Wang et al. 2009)
5-HT	Rat	Millipore (Temecula, CA, USA; cat. No. MAB352 clone YC5/45) RRID: AB_11213564	1:500	Staining absent when primary or secondary omitted. Staining pattern matches that of another well- validated 5-HT antibody (Kaya et al. 2004; Ma et al. 2007) and has been used previously (Mansouri-Guilani et al. 2019)
KRT8	Rat	University of Iowa Developmental Studies Hybridoma Bank (RRID: AB_531826)	1:500	Extensively used antibody, displays staining pattern consistent with that observed in taste tissues (Biggs et al. 2016)