Fig. 1.

The proteomic work flow applied in the study. a A spectral-library-based proteomic approach was used to compare the plasma proteomes between Good-responders and Limited-responders. Plasma samples were immunodepleted, digested and analyzed by LC–MS/MS. A spectral library was constructed for peptide/protein identification and quantification. b An example of peptide identification and quantification using spectral library based approach—the elution profile and MS/MS library matching of a doubly charged peptide DEPTYILNIK. The number of lines on the peptide elution profile indicates the number of the times the peptide has been fragmented and identified during the acquisition