Fig. 6.

PHLDB2 binds to MDM2 and facilitates MDM2-mediated E-Cadherin degradation. a HCT116 p53^−/− cells were transfected with HA-MDM2 with or without Flag-PHLDB2 and cell lysis was harvested for co-immunoprecipitation using anti-Flag antibody and Western Blot analysis at 48 h post-transfection. b HCT116 p53^−/− cells were lysed and subjected to co-immunoprecipitation using anti-MDM2 antibody or mouse IgG as negative control. c HCT116 p53^−/− cells were co-transfected with plasmids as indicated and subjected to Western Blot analysis at 48 h post-transfection. d Scheme depicts the role of PHLDB2 in EMT pathway. EMT stimuli, for example TGF-β or EGF, regulate multiple genes expression, such as Vimentin, E-Cadherin, and PHLDB2 in our study. PHLDB2 can bind to MDM2 to facilitate E-Cadherin degradation, and therefore promote EMT phenotypes