Fig. 4.

Overexpression of miR-34a reduced cell surface expression of CD11A on primary CD4⁺ or CD8⁺T cells and secretion of PRF1 from CD8⁺ T cells. CD4⁺ or CD8⁺ T cells were transfected either with allstars negative control (ANC) or miR-34a-5p mimic. a Mean fluorescence intensities of CD11A expression from ANC-transfected (red) or miR-34a-5p mimic-transfected (blue) CD4⁺ T cells or respective isotype controls (green and grey) was analyzed. b Mean fluorescence intensities of CD11A expression from ANC-transfected (red) or miR-34a-5p mimic-transfected (blue) CD8⁺ T cells or respective isotype controls (green and grey) was analyzed. c FACS data were summarized from three independent experiments performed in duplicates. Two asterisks correspond to p-value ≤0.01 and ≥ 0.001. Data are represented as mean ± SEM. d FACS data were summarized from three independent experiments performed in duplicates. Three asterisks correspond to p-value ≤0.001. Data are represented as mean ± SEM. e ELISA data were quantified from three independent experiments from two different donors. Three asterisks correspond to p-value ≤0.001. Data are represented as mean ± SEM