Figure 9.

ASIC1a induces PKCλ phosphorylation through intracellular Ca²⁺ increase. A, Acid (pH 6.0)-induced changes in cytosolic Ca²⁺ signal, indicated by GCaMP6 fluorescence, in cultured WT or ASIC1a KO mouse cortical neurons. n = 9 or 10. Thick lines indicate mean. Shaded areas represent SEM. Right, Representative images of GCaMP6-labeled neurons at indicated time points (I, II, and III). Scale bar, 10 μm. B, C, Representative images (B) and quantification (C) of Western blots showing that the acidic solution, pH 6.0, treatment elicited a significant increase in the phosphorylation of PKCλ in cultured cortical neurons from WT but not ASIC1a KO mice. A Ca²⁺-free pH 6.0 solution failed to evoke PKCλ phosphorylation. n = 4. D, E, Representative images (D) and quantification (E) of Western blots showing that chelating intracellular Ca²⁺ by BAPTA-AM also eliminated the acidic solution-induced PKC phosphorylation. n = 4. **p < 0.01, N.S., not significant.