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. 2019 Jul 17;39(29):5647–5661. doi: 10.1523/JNEUROSCI.0479-19.2019

Figure 3.

Figure 3.

NAc and DS CINs ubiquitously express CRF-R1. a, Representative fluorescent ISH images of CIN marker ChAT mRNA (red), mRNAs (green) for Crh1 (CRF-R1, top) or Crh2 (CRF-R2, bottom) and DAPI nuclear stain (blue) in NAc. b–c, Quantification of ChAT (CIN) colocalized with mRNAs for Crh1 (b), Crh2 (c), in NAc (left) or DS (right). d, Quantification of DAPI cells colocalized with mRNAs for Crh1 (left) and Crh2 (middle) in the NAc. The number of CRF-R1+, CRF-R2+, and ChAT+ cells per 100 μm2 (right). e, Quantification of DAPI cells colocalized with mRNAs for Crh1 (left) and Crh2 (middle) in the DS. The number of CRF-R1+, CRF-R2+, and ChAT+ cells per 100 μm2 (right). f, Example images of ChAT and Crh1 (left) or Crh2 (right) mRNA expression in the diagonal band of the basal forebrain. g, Quantification of ChAT and Crh1 (top) or Crh2 (bottom) mRNA expression in the diagonal band region of the basal forebrain using fluorescent ISH. h, Quantification of DAPI and Crh1 (top) or Crh2 (bottom) mRNA expression in the diagonal band region of the basal forebrain using fluorescent ISH.