Fig. 2.

Loss of mTOR and PI3K signaling blocks H3K9ac induced by insulin. a Western blot analysis using the indicated antibodies in MDA-MB-231 cell lysates treated with insulin (100 nM) for 1 h, 3 h or 6 h. b Western blot analysis using the indicated antibodies in MDA-MB-231 cells pretreated (lanes 4–6) or not (lanes 1–3) with 20 nM mTOR inhibitor rapamycin (1 h) followed by insulin treatment for 3 h. c Densitometric quantification of H3K9ac/H3 western blot signals in (b). Data are represented as fold change over respective UT. Values are Mean + SEM; n = 3. Statistical significance was calculated using one-way ANOVA, Tukey’s multiple comparisons test. **p < 0.01. d Western blot analysis using the indicated antibodies in MDA-MB-231 cells pretreated (lanes 3 and 4) or not (lanes 1 and 2) with 50-µM PI3K inhibitor LY294002 (1 h) followed by insulin treatment for 3 h