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. 2019 Jul 16;20:155. doi: 10.1186/s12931-019-1115-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Expression of biologic markers associated with lung and kidney damage. Real-time polymerase chain reaction analysis of biologic markers associated with lung inflammation (interleukin [IL]-6) (a), vascular integrity (VE-CAD) (b), and fibrosis (procollagen [PC]-III) (c), as well with renal injury (kidney injury molecule [KIM-1]) (d) and regeneration (nephronectin, NPNT) (e). Boxes show the interquartile range (25th–75th percentile), while whiskers encompass the range (minimum-maximum) and horizontal lines represent the median in 6 animals/group. Relative gene expression was calculated as a ratio of average expression of each gene to the reference gene (36B4) and expressed as fold change relative to non-ventilated animals (NV) and no fluid. Values are medians and interquartile ranges of 6 rats in each group. Comparisons among all groups were done by the Kruskal–Wallis test followed by Dunn’s test (p < 0.05)