Fig. 8.

The effect of drinking water containing trehalose on beige-adipocyte-related gene expression. Total RNAs were extracted from mesenteric adipose tissues and inguinal adipose tissues, and the respective cDNAs were prepared as described in the manual. Subsequently, real-time PCR was performed using a LightCycler®480 system. For quantitative purposes, expression levels of the respective target genes were normalized to the housekeeping gene 18S rRNA. Mesenteric adipose tissues (a) and inguinal adipose tissues (b) are shown. Values are shown as means ± standard deviations (n = 8). Cidea expression was significantly higher in the trehalose group compared with both the water and the maltose groups (8b; p < 0.05, Steel). Ucp1 expression was significantly higher in the trehalose group compared with the maltose group (8b; p < 0.05, Tukey-Kramer). Cidea: cell death-inducing DNA fragmentation factor-α-like effector A; UCP1: uncoupling protein 1