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. 2019 Jul 16;16:45. doi: 10.1186/s12986-019-0373-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 9 — Effect of drinking water containing trehalose on BAT. Histological analysis of BAT (a) hematoxylin-eosin staining, × 400). Arrows (→) indicate vacuolation of BAT cells by fat deposition. Fatty degeneration scores (b) were graded as negative: 0, slightly: 1, mildly: 2, and severe: 3. Subsequently, the personal score was plotted (n = 8). Statistical analysis was performed using the nonparametric Wilcoxon test. Values show statistical significance (9b; p < 0.01 and p < 0.05). BAT: brown adipose tissue