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. 2019 Jul 8;14:5033–5050. doi: 10.2147/IJN.S200782

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© 2019 Bacakova et al.

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Human dermal fibroblasts in the collagen hydrogel migrated from a non-coated membrane (Gel PLLA) and from a fibrin-coated membrane (Gel PLLA F) for 1, 3, 7, and 14 days, or human dermal fibroblasts proliferated on the non-coated membrane (PLLA beneath gel) or on the fibrin-coated membrane (PLLA F beneath gel) beneath the collagen hydrogel for 4, 7, 10, and 17 days. The fibrin was stained by immunofluorescence (Alexa 488, green). The cells were stained with phalloidin-TRITC (red; F-actin cytoskeleton) and with Hoechst #33,258 (blue; cell nuclei). Leica TCS SPE DM2500 confocal microscope, 20x/0.75 IMM CORR CS2 zoom 2x or obj. 40x/1.15 NA oil, maximal intensity projection images.

Abbreviation: PLLA, poly-L-lactide.