Figure 3.
Hepatitis A virus genome and targets used for sequence-based typing, European Union/European Economic Area countries, 2014 (n = 15 countries providing information) and 2016 (n = 17 countries providing information)
bp: base pair; kb: kilo bases; PHE: Public Health England; ssRNA: single stranded RNA; UTR: untranslated region; VP: viral protein.
a Belgium changed the region of the genome targeted for amplification/sequencing after 2016 and now uses the HAVNET protocol.
The ( + ) ssRNA (7.5 kb) consists of three open reading frames (P1–P3) encoding structural (P1) and non-structural proteins (P2, P3). P1 encodes the capsid polypeptides (VP1–4), and the P2 and P3 regions encode the non-structural polypeptides required for virus replication. The horizontal bars represent the position in the genome of the region amplified and sequenced by the respective countries in 2014 and 2016. The sizes of the respective regions after removing primer binding sites are indicated in parentheses next to the horizontal bars. The GenBank sequence NC_001489 (HAV, complete genome) was used as reference.
