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. 2019 Jun 9;10(16):3632–3638. doi: 10.7150/jca.33237

Figure 2.

Figure 2

LKB1 inhibits YAP nuclear localization by the phosphorylation of YAP in lung cancer lines. (A) The relative levels of LKB1, pYAP, YAP, GAPDH, and Histone were measured by western blotting. Cytoplasmic LKB1, pYAP and YAP expression levels were higher but nuclear YAP expression levels were lower in A549 cells and LK2 cells transiently expression LKB1. Cytoplasmic LKB1, pYAP and YAP expression levels were lower but nuclear YAP expression levels were higher in NCI-H460 cells transfected with LKB siRNA. (B) Immunofluorescence showed that LKB1 downregulated YAP nuclear localization while LKB1 siRNA upregulated YAP nuclear localization. Scale bar, 20μm. Mock: mock transfection; vector: empty vector; ns: no significance.