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. 2019 Jun 9;10(16):3778–3788. doi: 10.7150/jca.30359

Figure 4.

Figure 4

THZ1 protect rabbit primary lens epithelial cell from TGFβ2 induced EMT, influenced the phosphorylation of Smad2 and EMT signatures of LECs. (A) The down-regulation of Fibronectin, SNAI2, Collagen I was examined by RT-PCR after co-treated rabbit primary lens epithelial cell with TGFβ2 and THZ1 (0, 100, 200nM). Protein level of p-Smad2, Smad2 was detected by western blot with GAPDH as the internal control (B). C is the quantification of B. (D) Heat map analysis of the EMT signature in LECs co-treated with TGFβ2 (10ng/ml) and THZ1 (100nM) or DMSO for 24 h. (E) Influenced signaling pathways based on KEGG analysis from RNA-sequence. Results are presented as the mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 vs. Control.