Figure 2.

Upregulation of AHNAK was discovered in PDAC and PDAC cell lines. A and B: Sixty-six PDAC specimens were collected and subjected to immunohistochemical staining, which showed higher AHNAK protein expression in PDAC than in adjacent non-tumor tissues. C and D: AHNAK expression was measured in three pancreatic ductal adenocarcinoma cells(PANC-1, Mia PaCa-2 and AsPC-1) and Pancreatic ductal normal cells (hTERT-HPNE). The PANC-1 cells had higher AHNAK mRNA and protein expression levels as detected by qPCR and western blotting analyses.