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SIRT5 knockdown increases HCC cell apoptosis. A-D. After transfection with control siRNA, siRNA#1 and siRNA#2 in Bel and Huh7 cells, the apoptotic cells were stained with Annexin V-PI and detected by flow cytometry. E, F and G. The protein levels of cl-cas3, cl-PARP, Bax, Bcl-2 and SIRT5 were detected by western blotting. *P < 0.05, **P < 0.01.
