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SIRT5 knockdown induces mitochondrial apoptosis in HCC cells. A-D. After control siRNA, siRNA#1 and siRNA#2 transfection, JC-1 dye was used to stain Bel and Huh7 cells, and alterations in mitochondrial membrane potential were detected by flow cytometric analysis. E-J. Cyt c in cytoplasm and mitochondria of Bel and Huh7 cells was evaluated by western blot. *P < 0.05, **P < 0.01.
