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. 2019 Jun 9;10(16):3735–3745. doi: 10.7150/jca.32022

Figure 3.

Figure 3

BDH2 expression in different HCC cell lines and validation of BDH2 overexpression in transfected HCC cell lines. A. Lower expression levels of BDH2 mRNA were detected in HCC cell lines (HepG2, Hep3B, QGY-7703, HuH-7, MHCC-97L and MHCC-97H) than in normal L02 cells, according to the qRT-PCR assay. GAPDH was used as an internal quantitative control. B. BDH2 overexpression was successfully verified in transfected HCC cell lines (HepG2 and Hep3B) by qRT-PCR. C. BDH2 overexpression was successfully verified in transfected HCC cell lines (HepG2 and Hep3B) by western blot. All experiments were performed using vector-transfected cells as a control. Asterisks indicate statistically significant differences (*P<0.05, **P<0.01).