Table 1.

Physicochemical characteristics of unloaded (Nano.Alg) and miltefosine-loaded (MFS.Alg) alginate nanoparticles before and after the freeze-drying process

	Size (nm)	Pdi	Zeta potential (mV)
Nano.Alg before freeze-drying	335.7±10.9	0.35±0.06	−21.1±1.3
Nano.Alg after freeze-drying	346.5±30.6	0.40±0.07	−36.2±6.8*
MFS.Alg before freeze-drying	320.2±37.9	0.43±0.14	−19.0±3.1
MFS.Alg after freeze-drying	279.1±56.7	0.42±0.15	−39.7±5.2*

Notes: Data represent mean ± standard deviation. *P<0.05 compared with nanoparticles before freeze-drying (Student’s t-test).