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. 2019 Jul 12;14:5229–5242. doi: 10.2147/IJN.S210546

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© 2019 Kim et al.

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Effects of NP[OVA+Dex] on the expression of cell surface molecules in DCs.

Notes: (A) DCs generated from the bone marrow cells of C57BL/6 mice were stimulated with IFN-γ (50 ng/mL) plus TNF-α (50 ng/mL), or treated with NP[OVA] or NP[OVA+Dex] (10 μg/mL as OVA) for 48 h. DCs were stained for CD11c, H-2K^b, I-A^b, CD80, CD86, and PD-L1. CD11c⁺ cells were gated and analyzed for the expression of cell surface molecules. The data shown are representative histograms of four independent experiments. (B) Mean fluorescence intensities of NP-treated, or untreated DCs. The data are presented as the mean ± SD of four independent experiments. (C) Expression of CD86 and PD-L1 in NP-treated, or untreated DCs. The data shown are representative histograms of four independent experiments. (D) The proportion of CD86^loPD-L1^hi cells in each experimental group is shown. The data are presented as the mean ± SD of four independent experiments. The significance of the data was evaluated using a Tukey test of one-way ANOVA test. *P<0.05, **P<0.01, ***P<0.001. “ns” indicates no significant difference.

Abbreviations: DCs, dendritic cells; Dex, dexamethasone; IFN-γ, interferon-γ; NP, nanoparticle; NP[OVA+Dex], nanoparticles containing ovalbumin and dexamethasone; NP[OVA], nanoparticles containing only ovalbumin; OVA, ovalbumin; PD-L1, programmed death-ligand 1; SD, standard deviation; TNF-α, tumor necrosis factor-α.