Table 1.

Characterization of PLGA-NPs

	Particle size (nm)	Polydispersity index	OVA loading capacity(μg/mg NP)	Dex loading capacity (μg/mg NP)	Zeta-potential (mV)
NP[OVA]	863.23±62.89	0.125±0.045	148.26±11.20	－	−1.85±0.13
NP[OVA+Dex]	864.80±20.50	0.269±0.022	148.37±10.92	0.978±0.022	−1.79±0.56

Notes: The average size, polydispersity index, and zeta-potential of the NPs were measured using a particle size analyzer. The OVA content was determined using a microbicinchoninic acid assay after lysing the NPs in a lysis buffer containing 0.1% SDS and 0.1 N NaOH. The concentration of Dex was determined by high performance liquid chromatography using a YMC J’sphere ODS-H80 column (150×4.6 mm) after lysing the NPs in a 1:2 mixture of dimethyl sulfoxide and methanol.

Abbreviations: Dex, dexamethasone; NPs, nanoparticles; NP[OVA+Dex], nanoparticles containing ovalbumin and dexamethasone; NP[OVA], nanoparticles containing only ovalbumin; OVA, ovalbumin; SDS, sodium dodecyl sulfate.