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. Author manuscript; available in PMC: 2019 Jul 17.
Published in final edited form as: Fertil Steril. 2009 Jun 12;93(8):2687–2694. doi: 10.1016/j.fertnstert.2009.04.042

Figure 2.

Figure 2

Figure 2

(A) The human VEGF gene is overexpressed in ectopic endometriosis. RNA was extracted from deep infiltrating endometriotic lesions, eutopic endometrium and normal peritoneum from four patients suffering from deep infiltrating endometriosis and reverse-transcribed into cDNA. Real-time PCR analysis was performed to quantify the expression of the VEGF gene. The gene copy numbers are normalized by the GAPDH copy number. Error bars indicate SD. *p<0.05 versus eutopic EM and Peritoneum

(B) The human VEGF promoter is active in endometriotic lesions. Ectopic endometrium cells from three women with endometriosis and eutopic endometrium from three healthy patients were infected with AdVEGFluc and AdCMVluc, respectively at an MOI of 10. Luciferase activity of the VEGF promoter is expressed as relative light units (RLU) as a percentage of the CMV promoter driven activity. Each point represents the mean of three experiments. Error bars indicate standard deviation. *p<0.05 versus eutopic endometrium