Figure 3.
(A) Induction of apoptosis in endometriosis cells after infection with the VEGF targeted CRAD. Endometriosis cells were infected at an MOI of 50 pfu/cells with Ad5VEGFE1, AdVEGFluc or no virus. Apoptosis was detected by TUNEL assay. Each bar represents the mean of three experiments. Error bars indicate standard deviation.
(B) Ad5VEGFE1 replicates in purified human endometriotic cells. Purified and unpassaged endometriotic cells were infected with 1000 vp/cell of Ad5VEGFE1, Ad5wt (wild type) and AdVEGFluc. Cells and growth medium were collected at indicated time points and virus copy number was measured with quantitative PCR.