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. Author manuscript; available in PMC: 2020 Jun 19.
Published in final edited form as: Biochem J. 2019 Jun 19;476(12):1713–1724. doi: 10.1042/BCJ20190302

Figure 5: GCN5L1 reduces Rictor/mTORC2 acetylation.

Figure 5:

A. Co-Immunoprecipitation of Rictor with GCN5L1 from wildtype mouse cardiac tissues. B. Immunoprecipitation of Rictor from AC16 cells using anti acetyl-lysine antibody. N = 5-6 samples per genotype and condition. C. Mitochondrial ROS production of GCN5L1 knockdown cells after transfection with vector (vec), pseudoacetylated Rictor (3KQ) or acetylation incompetent Rictor (3KR). N = 24 wells per transfection. D. Survival of wildtype and GCN5L1 knockdown cells after transfection with vector, 3KQ Rictor, or 3KR Rictor, subjected to 3 hours of hypoxia and 1 hour of reoxygenation. N = 20 wells per transfection and condition. Norm = Normoxia, H/R = Hypoxia/reoxygenation. *p<0.05 vs control.