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. Author manuscript; available in PMC: 2019 Jul 17.
Published in final edited form as: Science. 2019 Feb 22;363(6429):eaav7969. doi: 10.1126/science.aav7969

Figure 1: Latrophilin-3 (Lphn3) is essential for formation of a subset of excitatory synapses in cultured hippocampal neurons.

Figure 1:

A. Schematic of the Lphn3 domain structure with location of the HA-tag in Lphn3 cKO mice.

B. Lphn3 is postsynaptic as revealed by sparse expression of Cre-recombinase in transfected hippocampal neurons that were cultured from Lphn3 cKO mice.

C-E. Postsynaptic Lphn3 deletion in cultured hippocampal neurons decreases the density of dendritic spines and the number of excitatory but not of inhibitory synapses.

F-I. Postsynaptic Lphn3 deletion suppresses the frequency of spontaneous mEPSCs but not mIPSCs monitored in the presence of tetrodotoxin.

J & K. Postsynaptic deletion of Lphn3 decreases the amplitudes of evoked EPSC but not IPSCs.

Data are means ± SEM (numbers of cells/experiments are indicated in bars). Statistical significance was assessed by two-tailed t-tests (** denotes p<0.01; * denotes p<0.05).