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. Author manuscript; available in PMC: 2019 Jul 17.
Published in final edited form as: Science. 2019 Feb 22;363(6429):eaav7969. doi: 10.1126/science.aav7969

Figure 4: Lphn3 is selectively essential for Schaffer-collateral synapse formation in the CA1 region.

Figure 4:

A. Unilateral infection of the neonatal hippocampal CA1 region with lentiviruses expressing EGFP-tagged Cre-recombinase causes sparse deletion of Lphn3 in Lphn3 cKO mice. Image shows a hippocampal section stained for DAPI; infected neurons are identified via their EGFP fluorescence.

B. Filling of individual neurons with biocytin via a patch pipette enables analysis of dendritic morphology. Image shows representative pyramidal neuron filled with biocytin; Cre-recombined and uninfected control neurons were examined in opposite hemispheres of the same mice.

C-E. Quantifications of biocytin-filled, patched CA1 neurons show that postsynaptic Lphn3 deletion decreases dendritic spine densities in the S. oriens and S. radiatum but not the S. lacunosum-moleculare.

F. Diagram of the electrophysiological recording configuration in acute slices with sparse lentiviral expression of Cre-recombinase.

G-I. Postsynaptic Lphn3 deletion in CA1 region neurons decreases the mEPSC but not mIPSC frequency (G, representative mEPSC and mIPSC traces; H & I, summary graphs of the mean mEPSC (H) and mIPSC frequency (I)).

J. Postsynaptic Lphn3 deletion in CA1 neurons decreases the Schaffer-collateral synaptic strength (left, representative traces; right, summary graph of EPSC amplitude). Input-output curves were used to control for differences in stimulus strength.

K. Postsynaptic Lphn3 deletion in CA1 neurons had no effect on the synaptic strength of entorhinal cortex inputs (left, representative traces; right, summary graph of EPSC amplitude).

L. Measurements of paired-pulse ratios of Schaffer-collateral EPSCs as a function of the interstimulus interval show that the Lphn3 deletion has no effect on release probability (left, representative traces; right, summary plot).

M. Same as J, but measured in acute slices from mice that were sparsely infected with Cre-expressing lentiviruses at P21, and analyzed at P40.

Numerical data are means ± SEM (numbers of cells/mice are indicated in bars). Statistical significance was assessed by two-tailed t-tests (** denotes p<0.01; * denotes p<0.05).