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. Author manuscript; available in PMC: 2020 Jun 6.
Published in final edited form as: Mol Cell. 2019 May 7;74(5):982–995.e6. doi: 10.1016/j.molcel.2019.04.006

Figure 2. Phased piRNA production promotes nuage localization of Armi.

Figure 2.

(A) Immunofluorescence co-detection of Armi and Aub, Ago3, Vasa, Zuc-3×FLAG or ATP5A in wild-type or zucH169Y stage 3 egg chambers or of Armi and Aub or Zuc-3×FLAG in minotaurz3-5967 stage 3 egg chambers. Box plots show the fraction of Aub signal overlapping with Armi in wild-type, zucH169Y or minotaurz3-5967 stage 3 egg chambers. Box comprises the 25th percentile and the 75th percentile of data, with the median indicated; whiskers mark 1.5 × interquartile range. p-value was calculated using the two-tailed Mann Whitney test.

(B) Scatter plots of the abundance of transposon- or gene-mapping 5′ monophosphorylated RNA >150 nt co-immunoprecipitated with Armi or control in wild-type or zucH169Y mutant ovaries.

See also Figure S2–S4.