Table 1.
All data are mean ± S.D. for weighted spectra count (n = 3) and FDR < 0.05 for experimental immunoprecipitate (IP) versus control.
| (A) piRNA biogenesis proteins specifically co-immunoprecipitated with Zuc, Armi or ArmiK729A. See also Table S1. | |||||||
|---|---|---|---|---|---|---|---|
| Protein | Localization | Zuc IP | Control IP for Zuc | Armi IP | ArmiK729A IP | Control IP for Armi | |
| Zucchini (Zuc) | Mitochondria | 31 ± 6 | 0 | – | – | – | |
| Gasz | Mitochondria | 15 ± 5 | 0 | 7 ± 2 | 8.3 ± 0.6 | 0 | |
| Papi | Mitochondria | 11 ± 1 | 0 | – | – | – | |
| Armitage (Armi) | Mitochondria & nuage | 38 ± 3 | 5 ± 4 | 200 ± 90 | 180 ± 80 | 10 ± 20 | |
| Minotaur (Mino) | Mitochondria & ER | 16 ± 4 | 0.7 ± 0.6 | 11 ± 5 | 14 ± 2 | 0.3 ± 0.6 | |
| Sister of Yb (SoYb) | Mitochondria? | 4 ± 4 | 0 | 10 ± 10 | 20 ± 10 | 0 | |
| Vreteno (Vret) | Nuage | – | – | 4 ± 5 | 4 ± 5 | 0 | |
| Shutdown (Shu) | Nuage | – | – | 8 ± 4 | 16 ± 5 | 1 ± 1 | |
| Argonaute 3 (Ago3) | Nuage | – | – | 7 ± 7 | 4 ± 5 | 0.3 ± 0.6 | |
| Spindle E (Spn-E) | Nuage | – | – | 9 ± 9 | 9 ± 9 | 1 ± 2 | |
| Tapas | Nuage | – | – | 8 ± 4 | 8 ± 2 | 2 ± 2 | |
| Aubergine (Aub) | Nuage | – | – | 40 ± 20 | 40 ± 20 | 10 ± 7 | |
| Brother of Yb (BoYb) | Nuage | – | – | 5 ± 6 | 3 ± 4 | 0.3 ± 0.6 | |
| Nibbler (Nbr) | Nuage | – | – | 10 ± 4 | 14 ± 3 | 3.3 ± 0.6 | |
| Qin | Nuage | – | – | 5 ± 7 | 2 ± 4 | 0 | |
| P-element induced wimpy testis (Piwi) | Nucleus | – | – | 16 ± 6 | 20 ± 10 | 9 ± 6 | |
| Vasa (Vas) | Nuage | – | – | 26 ± 6 | 33 ± 2 | 14 ± 2 | |
| Tudor (Tud) | Nuage | – | – | 40 ± 30 | 30 ± 30 | 20 ± 20 | |
| (B) Proteins not known to function in piRNA biogenesis that co-immunoprecipitated with Zuc | |||||||
| Mitochondrial Proteins | Others | ||||||
| Fly name | Human name | Zuc IP | Control IP | Fly name | Human name | Zuc IP | Control IP |
| Miro | Rhot1 | 10 ± 3 | 0 | CG12360 | Trabd | 12 ± 5 | 0 |
| Miga | Miga2 | 11 ± 2 | 1 ± 1 | CG10880 | – | 11 ± 3 | 0.3 ± 0.6 |
| CG9855 | March5 | 5 ± 2 | 0 | nmd | Atad1 | 16 ± 6 | 1 ± 1 |
| Spoon/Yu | Akap1 | 10 ± 4 | 0.7 ± 0.6 | Aldh-III | Aldh3b1 | 9 ± 2 | 0 |
| Marf | Mfn2 | 27 ± 4 | 2 ± 2 | CG7705 | – | 10 ± 3 | 0 |
| Usp30 | Usp30 | 3 ± 4 | 0 | Faf | Faf2 | 7 ± 5 | 0 |
| Men | Me1 | 13 ± 9 | 1 ± 2 | Pex3 | Pex3 | 5 ± 1 | 0.3 ± 0.6 |
| Mtch | Mtch2 | 12 ± 6 | 2 ± 2 | CG2316 | Abcd2 | 5 ± 2 | 0 |
| Ptp61F | Ptpn1 | 5 ± 1 | 0.3 ± 0.6 | Myt1 | PKMYT1 | 4 ± 2 | 0 |
| CG7639 | SAMM50 | 4 ± 3 | 0 | CG1291 | Alg2 | 5 ± 3 | 0.3 ± 0.6 |
| CG1665 | Marc1 | 15 ± 7 | 1.3 ± 0.6 | CG8735 | LNP | 6.3 ± 0.6 | 0.3 ± 0.6 |
| CG7461 | Acadvl | 3 ± 1 | 0 | CG6550 | CDKAL1 | 4 ± 3 | 0 |
| Tom70 | Tomm70a | 28 ± 7 | 5 ± 1 | Sec63 | Sec63 | 7 ± 3 | 1 ± 2 |
| CG3394 | SLC27A1 | 5 ± 4 | 1 ± 1 | CG9853 | Get4 | 5 ± 3 | 0.3 ± 0.6 |
| Pmp70 | Abcd3 | 11 ± 5 | 2 ± 3 | CG6744 | Exd2 | 3 ± 4 | 0 |
| iPLA2-VIA | Pla2g6 | 7 ± 3 | 1.3 ± 0.6 | CG7546 | Bag6 | 10 ± 6 | 2 ± 1 |
| Acsl | Acsl3 | 17 ± 2 | 7 ± 2 | CG6089 | Tmem214 | 13 ± 6 | 2 ± 1 |