FIGURE 6.

Structures of negative strand RNA viral RdRp domains. (A) Partial amino acid sequences containing RdRp motifs A–F of the VSV L protein are shown with their secondary structures (cylinders, α-helices; arrows, β-strands). The catalytic aspartate residues are indicated by red arrowheads. Amino acid sequence logos for RdRp motifs A–F in L proteins of 231 NNS RNA viruses belonging to the Rhabdoviridae, Paramyxoviridae, Filoviridae, Bornaviridae, and Nyamiviridae families (Maes et al., 2019) were generated using the WebLogo program (Crooks et al., 2004)² as described in Neubauer et al. (2016). (B–D) The three-dimensional structural model of the RdRp domain of VSV (B) is compared with those of influenza A virus [FLUAV, PDB id: 4WSB (Pflug et al., 2014)] (C), and LACV [PDB id: 5AMQ (Gerlach et al., 2015)] (D). Their fingers, palm, and thumb subdomains are colored as in Figure 5A. The positions of RdRp motifs A–F are indicated by circled letters. The priming-capping loop of the VSV PRNTase domain and priming loops extended from the C-terminal regions of the FLUAV and LACV thumb subdomains are shown in orange. Missing regions of the loops in the structures are denoted by orange dashed lines. Other virus-specific substructures in their fingers subdomains are colored gray.